What amounts went into each test
TB-500 dosage and missing human facts
Animals supplied most amounts. One human test used the full protein. Neither one sets a sound TB-500 dose for you.
What TB-500 study amounts were meant to test
Animal researchers chose amounts to answer study questions. They weren't setting a human TB-500 plan. Most animals got full thymosin beta-4. One Phase 1 human safety test used thymosin beta-4 too. The notes below name the animal, shot route, and amount. They aren't dosing advice for you.
Rodent heart and brain tests used about 6–12 milligrams per kilogram. A heavier animal therefore got more. The rat stroke test gave belly shots ranging from 2–18 milligrams per kilogram [7]. The weak-muscle mice received 150 micrograms by belly shot. For six months, shots were given on two days of each week [8]. Skin cells in a dish moved after 10 trillionths of a gram [2]. That doesn't give a body dose. In the Phase 1 human test, people got 42, 140, 420, or 1260 milligrams of thymosin beta-4 by vein. One group received a single dose. Another received daily doses for 14 days [4].
What is known about how long TB-500 lasts
No sound human half-life is known for TB-500. Half-life is the time an amount needs to drop by half. In the Phase 1 vein test, full thymosin beta-4 lasted longer at larger amounts [4]. Horse-racing labs used chemical tests to find TB-500 and broken pieces [4]. Those tests show what a lab can spot. They don't show how a person's body clears the substance.
TB-500 has a small chemical change at one end. That change may slow how fast it breaks down. The chain can still break apart. Freezing and thawing may harm it too. Labs mix dry powder with clean water and keep it cold. None of this proves a half-life in you.
What a TB-500 drug test can tell you
No sound clearing time has been measured in people for TB-500 [4]. Horse-racing labs made chemical tests to find the substance. The tests find the substance and remnants made as it breaks down [4]. A trace only shows that the lab found something. It doesn't show when your body has cleared it.
The Phase 1 human test used the full protein. Its half-life rose with the amount [4]. In other words, larger amounts took longer to fall by half. That doesn't give a TB-500 clearing time. Exact times online didn't come from these studies.
What TB-500 loading and maintenance mean online
Online writers use "loading" for larger amounts at first. They use "maintenance" for smaller amounts later. No fair human trial has tested that plan [4]. Published amounts came from animals given full thymosin beta-4. Mice got 150 micrograms by belly shot twice a week [8]. Belly-shot amounts in stroke tests ranged from 2–18 milligrams per kilogram [7]. At 18 milligrams per kilogram, rats got no benefit [7]. More wasn't better. Animal amounts don't set your weekly plan.
What each TB-500 shot route has tested
Most rodent tests put the shot in the belly [7][8]. Online writers say they used skin or muscle shots themselves. Those are personal reports, not controlled study results. No fair human test compared the routes. In the Phase 1 safety test, people received the full protein through a vein [4]. They didn't get TB-500. Animal work can't tell you which route lasts longer.
Why TB-500 studies don't set your schedule
No sound human half-life has been found for TB-500 [4]. In the Phase 1 vein test, larger amounts of thymosin beta-4 lasted longer [4]. That test used the full protein. Online schedules haven't faced a fair human trial. One animal test also found that more didn't work better [7]. The research can't tell you how often to use the compound.